Paschalis Kratsios, PhD

My lab uses the specific strengths of two model systems (C.elegans and mice) to reveal the gene regulatory mechanisms that control motor neuron development and function. To reveal such mechanisms we employ novel methodology, such as whole genome sequencing, CRISPR genome editing, ATAC-seq and cell type-specific transcriptome profiling. Our laboratory aims to systematically test whether the function of the gene regulatory factors we discover in C.elegans is conserved across phylogeny using mouse genetics and novel genomic approaches.



A detailed understanding of how the motor neurons develop and function may provide novel entry points into the etiology, diagnosis or treatment of motor neuron disorders, such as spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). From the basic science perspective, our research will reveal novel transcription factors, their targets and the cis-regulatory elements (motifs) through which these factors act. Such decoding of cis-regulatory information is a vital step toward understanding genome function.

Columbia University
Postdoctoral training - Developmental Neurobiology
2016

European Molecular Biology Laboratory (EMBL)
Ph.D. - Developmental Biology
2009

Control of spinal motor neuron terminal differentiation through sustained Hoxc8 gene activity.
Catela C, Chen Y, Weng Y, Wen K, Kratsios P. Control of spinal motor neuron terminal differentiation through sustained Hoxc8 gene activity. Elife. 2022 Mar 22; 11.
PMID: 35315772

Emerging Roles for Hox Proteins in the Last Steps of Neuronal Development in Worms, Flies, and Mice.
Feng W, Li Y, Kratsios P. Emerging Roles for Hox Proteins in the Last Steps of Neuronal Development in Worms, Flies, and Mice. Front Neurosci. 2021; 15:801791.
PMID: 35185450

The SWI/SNF chromatin remodeling assemblies BAF and PBAF differentially regulate cell cycle exit and cellular invasion in vivo.
Smith JJ, Xiao Y, Parsan N, Medwig-Kinney TN, Martinez MAQ, Moore FEQ, Palmisano NJ, Kohrman AQ, Chandhok Delos Reyes M, Adikes RC, Liu S, Bracht SA, Zhang W, Wen K, Kratsios P, Matus DQ. The SWI/SNF chromatin remodeling assemblies BAF and PBAF differentially regulate cell cycle exit and cellular invasion in vivo. PLoS Genet. 2022 01; 18(1):e1009981.
PMID: 34982771

A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.
Sonobe Y, Aburas J, Krishnan G, Fleming AC, Ghadge G, Islam P, Warren EC, Gu Y, Kankel MW, Brown AEX, Kiskinis E, Gendron TF, Gao FB, Roos RP, Kratsios P. A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation. Nat Commun. 2021 10 15; 12(1):6025.
PMID: 34654821

Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf.
Li Y, Kratsios P. Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf. MicroPubl Biol. 2021; 2021.
PMID: 34549172

Tissue-specific targeting of DNA nanodevices in a multicellular living organism.
Chakraborty K, Anees P, Surana S, Martin S, Aburas J, Moutel S, Perez F, Koushika SP, Kratsios P, Krishnan Y. Tissue-specific targeting of DNA nanodevices in a multicellular living organism. Elife. 2021 07 28; 10.
PMID: 34318748

Establishment and maintenance of motor neuron identity via temporal modularity in terminal selector function.
Li Y, Osuma A, Correa E, Okebalama MA, Dao P, Gaylord O, Aburas J, Islam P, Brown AE, Kratsios P. Establishment and maintenance of motor neuron identity via temporal modularity in terminal selector function. Elife. 2020 10 01; 9.
PMID: 33001031

Modular Organization of Cis-regulatory Control Information of Neurotransmitter Pathway Genes in Caenorhabditis elegans.
Serrano-Saiz E, Gulez B, Pereira L, Gendrel M, Kerk SY, Vidal B, Feng W, Wang C, Kratsios P, Rand JB, Hobert O. Modular Organization of Cis-regulatory Control Information of Neurotransmitter Pathway Genes in Caenorhabditis elegans. Genetics. 2020 07; 215(3):665-681.
PMID: 32444379

A terminal selector prevents a Hox transcriptional switch to safeguard motor neuron identity throughout life.
Feng W, Li Y, Dao P, Aburas J, Islam P, Elbaz B, Kolarzyk A, Brown AE, Kratsios P. A terminal selector prevents a Hox transcriptional switch to safeguard motor neuron identity throughout life. Elife. 2020 01 03; 9.
PMID: 31902393

Transcriptional mechanisms of motor neuron development in vertebrates and invertebrates.
Catela C, Kratsios P. Transcriptional mechanisms of motor neuron development in vertebrates and invertebrates. Dev Biol. 2021 07; 475:193-204.
PMID: 31479648

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Award for Basic Research in Neurobiology
Whitehall Foundation
2017 - 2019

K99/R00 Pathway to Independence Award
National Institute of Health
2013 - 2018